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Table 2 Summary and comparison of pre-miRNA base pairing and nucleotide composition

From: Correlation between sequence conservation and structural thermodynamics of microRNA precursors from human, mouse, and chicken genomes

Species

Conservation

bp %

(A-U) %

(A+U) %

A %

U %

G %

C %

Human

S n (132)

68.66 ± 7.83

42.73 ± 16.12

47.11 ± 13.18

22.10 ± 7.89

25.01 ± 6.96

27.49 ± 7.90

25.40 ± 7.24

 

S c 1 (60)

71.88 ± 6.20A

49.34 ± 11.81Ab

53.87 ± 10.66A

23.78 ± 6.36

30.09 ± 6.42A

24.45 ± 5.53AB

21.68 ± 5.84A

 

S c 2 (66)

72.94 ± 5.64Ab

48.67 ± 8.17AB

51.62 ± 7.15AB

23.41 ± 4.74B

28.21 ± 4.42AB

25.71 ± 3.49aB

22.67 ± 5.11AB

 

S c 3 (33)

73.48 ± 4.67AB

50.14 ± 11.02Ab

52.94 ± 9.67a

22.96 ± 6.05

29.98 ± 6.16A

26.06 ± 5.15B

20.99 ± 5.92A

Mouse

S n (108)

67.87 ± 6.51

44.35 ± 11.20

49.30 ± 9.65

22.40 ± 6.16

26.90 ± 5.97

27.28 ± 5.57

23.42 ± 5.74

 

S c 1 (63)

72.53 ± 5.85A

48.20 ± 11.47a

53.07 ± 9.42a

23.29 ± 5.42

29.78 ± 6.11A

25.30 ± 5.28a

21.62 ± 5.16a

 

S c 2 (75)

72.10 ± 5.87A

47.70 ± 8.14aB

50.57 ± 6.77B

22.90 ± 4.57b

27.67 ± 4.40b

26.28 ± 3.72B

23.15 ± 5.02

 

S c 3 (39)

73.37 ± 5.13A

50.85 ± 10.93A

53.05 ± 9.15a

22.89 ± 5.90

30.16 ± 5.51A

26.06 ± 5.06

20.90 ± 5.53a

Chicken*

S n (21)

68.24 ± 7.37

37.13 ± 17.43

44.17 ± 14.75

19.47 ± 8.79

24.69 ± 8.01

29.35 ± 6.00

26.48 ± 9.74

 

S c 2 (40)

70.89 ± 7.06

51.22 ± 7.19AB

55.11 ± 4.99AB

24.60 ± 4.14B

30.52 ± 3.97AB

24.80 ± 3.53AB

20.08 ± 3.24aB

 

S c 3 (19)

73.08 ± 5.14a

53.80 ± 7.56AB

57.18 ± 5.72AB

24.45 ± 5.26

32.74 ± 3.26AB

23.94 ± 4.49A

18.87 ± 3.33AB

  1. Results represent the mean (± SD) of detailed information of base pairing and nucleotide composition. The number of pre-miRNAs within each set is indicated in brackets behind set name. * S c 1 for chicken was excluded as it contained fewer than five pre-miRNAs. Pair-wise comparisons of means (two-sided t-test) and variances (F-test) were performed between non-conserved (S n ) and conserved pre-miRNAs (S c 1, S c 2, and S c 3) from human, mouse, and chicken genomes. a mean value significantly different from that of S n at the 0.05 FDR level. A mean value is significantly different from that of S n at the 0.01 FDR level. b variance is significantly different from that of S n at the 0.05 FDR level. B variance significantly different from that of S n at the 0.01 FDR level.