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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Evaluating the feasibility of using candidate DNA barcodes in discriminating species of the large Asteraceae family

Figure 2

Analyses of the inter-specific divergence between congeneric species and intra-specific variation of the five loci. First, three parameters were used to characterize inter-specific divergence: (i) average inter-specific distance (K2P distance) between all species in each genus with at least two species; (ii) average theta prime (θ'), where theta prime is the mean pairwise distance within each genus with more than one species, thus eliminating biases associated with different numbers of species among genera; and (iii) smallest inter-specific distance, i.e., the minimum inter-specific distance within each genus with at least two species. Second, three additional parameters were used to determine intra-specific variation: (i) average intra-specific difference (K2P distance), that between all samples collected within each species with more than one individual; (ii) theta (θ), where theta is the mean pairwise distance within each species with at least two representatives; θ eliminates biases associated with unequal sampling among a species; and (iii) average coalescent depth, which is the maximum intra-specific distance within each species with at least two individuals.

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