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Table 1 Summary of the different analyses conducted

From: On the origin of Acochlidia and other enigmatic euthyneuran gastropods, with implications for the systematics of Heterobranchia

Sequence alignment/masking/phylogenetic analysis Model of sequence evolution Length of alignment Monophyly of Acochlidia and sister group relationship Changes within the tree topology compared to Figure 1
MAFFT + Gblocks + RAxML GTRCAT GTR + GAMMA 3641 bp see Figure 1 see Figure 1
MAFFT + ALISCORE + RAxML GTRCAT GTR + GAMMA 3926 bp Acochlidia monophyletic (no BS support) Aitengidae basal within Hedylopsacea; Acochlidia sister to (Hygrophila + (Glacidorboidea + (Amphiboloidea + Pyramidelloidea))) (no BS support) Anaspidea non-monophyletic; different internal topology of Cephalaspidea s.s. (Philine exigua basal to remaining taxa); Siphonarioidea and Sacoglossa form no clade, but Siphonarioidea + (Sacoglossa + remaining pulmonate taxa)
MAFFT + Gblocks + MrBayes GTR + G + I 3641 bp Acochlidia monophyletic (no significant PP); sister group to Eupulmonata (0.96 PP) basal tritomy within Euthyneura: (Acteonoidea + Rissoelloidea)/Nudipleura/remaining Euthyneura; different internal topology of Cephalaspidea s.s. (Philine exigua basal to remaining taxa), ((Glacidorboidea + Amphiboloidea) + Pyramidelloidea)
MAFFT + ALISCORE + MrBayes GTR + G + I 3926 bp Acochlidia monophyletic (no significant PP) Aitengidae basal within Hedylopsacea; Acochlidia sister to (Hygrophila + (Glacidorboidea + Amphiboloidea + Pyramidelloidea)) (no significant PP) Anaspidea non-monophyletic; different internal topology of Cephalaspidea s.s. (Philine exigua basal to remaining taxa); Siphonarioidea and Sacoglossa form no clade, but Siphonarioidea + (Sacoglossa + remaining pulmonate taxa); Nudipleura form a basal clade with (Acteonoidea + Rissoelloidea)
  1. The table lists the different methods of masking the alignment, phylogenetic approaches and models of sequence evolution used for the different analyses, as well as the resulting differences in tree topology (bootstrap support = BS; posterior probability = PP).