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Table 2 Primer pairs and annealing temperatures successfully used for primer walking.

From: The phylogenetic position of Acoela as revealed by the complete mitochondrial genome of Symsagittifera roscoffensis

Primer Pair Primer Sequence(3'-5') Annealing Temperature(°C)
SR 16S-f CTT ATG TTT TTT TTA GTT TGC GAC CTC 62
SR cob-f GGG GGA GTG ATT GCT TTG TTG C 64
SR cox3-f CAA CAG GGT TTC ACG GAA TAC ACG 65
SR 16S-r GAG GTC GCA AAC TAA AAA AAA CAT AAG 62
SR cobf-rev TGT TGG AGA TCT AAC AGA ATA AGC AC 60.1
SR co1f-rev CTT TCT GAG ATA AAA GTA GGT CCT GG 61.6
  1. Sequences are illustrated from 5' to 3' end.