Unusual conservation among genes encoding small secreted salivary gland proteins from a gall midge

Table 3 Evidence for positive selection on different alleles (Additional file 6, Figure S6)

Gene	Total cDNAs sequenced				Unique	Unique	Nonsyn/Syn
	GP	L	S	Total	cDNAs	proteins	SPCR	MPCR
*SSSGP-1A1*	60	43	45	148	71	27	0.83 (5/6)	1.56 (28/18)
*SSSGP-1B1*	106	24	36	166	66	35	0.80 (4/5)	1.50 (21/14)
*SSSGP-1C1*	27	5	12	44	20	14	0.60 (3/5)	2.33 (21/9)
RPs	157	34	23	214	96	47	N/A	0.26 (50/195)

cDNAs were derived from three Hessian fly populations: biotype GP, biotype L, and a Syrian population (S). As a control, cDNAs coding for 26 different ribosomal proteins (RPs) that were isolated along with SSSGP cDNAs were included in this analysis. Sequence alignments for different SSSGP cDNAs are shown in Additional file 6, Figure S6 whereas alignments for RP cDNAs are shown in Additional file 7, Figure S7. "Nonsyn/Syn" represents the ratios of non-synonymous against synonymous substitutions in SPCR and MPCR, respectively.

ISSN: 2730-7182