Figure 5
Summary of rDNA repeat ratios analysis estimated for individual synthetic T. mirus (A, C) and T. miscellus (B, D) plants. Homeolog gene ratios were determined from quantification of band signals after the Southern blot hybridization (Figures 2, 3). Each parental hybridization band was quantified using phosphorimaging, and gene ratios were expressed as a percentage of T. dubius units out of the total signal (A, B). The averaged values for each line are shown in graphs (C and D - shaded bars); for F1 diploid hybrids the shaded bars are drawn at low contrast. Filled bars represent expected gene ratios based on strict Mendelian inheritance of gene copy numbers. The interindividual variability within the line is expressed by standard deviation from the mean. Differences between expected and observed ratios were highly significant (P < 0.001, standard chi-square test) in all lines except the diploid F1-47 and F1-2 hybrids and line 121.