Table 1 Primers used in the study. F, forward; R, reverse.
From: Ancient origin of a Western Mediterranean radiation of subterranean beetles
Gene | Name | Sense | Sequence | Reference |
|---|---|---|---|---|
cox1 | Jerry (M202) | F | CAACATTTATTTTGATTTTTTGG | [72] |
| Â | Pat (M70) | R | TCCA(A)TGCACTAATCTGCCATATTA | [72] |
| Â | Chy | F | T(A/T)GTAGCCCA(T/C)TTTCATTA(T/C)GT | A. Cieslak, this work |
| Â | Tom | R | AC(A/G)TAATGAAA(A/G)TGGGCTAC(T/A)A | A. Cieslak, this work |
| Â | Tom-2 | R | A(A/G)GGGAATCATTGAATAAA(A/T)CC | A. Cieslak, this work |
cob | CB3 | F | GAGGAGCAACTGTAATTACTAA | [73] |
| Â | CB4 | R | AAAAGAAA(AG)TATCATTCAGGTTGAAT | [73] |
rrnL-nad1 | 16saR (M14) | F | CGCCTGTTTA(A/T)CAAAAACAT | [72] |
| Â | 16Sa | R | ATGTTTTTGTTAAACAGGCG | [72] |
| Â | 16Sb | R | CCGGTCTGAACTCAGATCATGT | [72] |
| Â | 16SAlf1 | R | GCATCACAAAAAGGCTGAGG | [74] |
| Â | ND1A (M223) | R | GGTCCCTTACGAATTTGAATATATCCT | [72] |
| Â | 16Sbi | F | ACATGATCTGAGTTCAAACCGG | [72] |
| Â | FawND1 | R | TAGAATTAGAAGATCAACCAGC | [72] |
SSU | 5' | F | GACAACCTGGTTGATCCTGCCAGT | [32] |
| Â | b5.0 | R | TAACCGCAACAACTTTAAT | [32] |
LSU | Ka | F | ACACGGACCAAGGAGTCTAGCATG | NHM (2000) |
| Â | Kb | R | CGTCCTGCTGTCTTAAGTTAC | NHM (2000) |