Figure 6

The analysis of substrate transport of MCF. (A) The logo chart of three tandem concatenated motifs of MCF for metazoan evolution. Three motif sequences constituting the conical pit were united end to end to form a mark sequence to research the variation in the substrate transported by MCF. WebLogo [21] was applied into the logo analysis. VER-MCF indicates a mark sequence of MCF in vertebrates. NONVER-MCF indicates a mark sequence of MCF in invertebrates. (B) Evolutionary pattern of substrate transport of MCs. A phylogenetic tree was constructed using MEGA4.0 [26] (Bootstrap = 1000, Poisson correction) based on TR sequences from 44 carriers in SLC25 and their orthologous sequences in nine species (44 Χ 9). A represents a subfamily. The index in SLC25 (the name of MCF in human) was used to denote the subfamily, such as A7, 8, 9 (representing the orthologous sequences of UCP1, 2, 3 in human, respectively). A47 represents the subfamily of Hepatocellular carcinoma-down-regulated mitochondrial carrier protein in human. The red branch indicates the carriers related to H⁺. Bootstrap values greater than 50 for selected nodes with clusters including more than four subfamilies were indicated.