Table 6 Description of PCR programs.

Std-Fix

3' 94°; 35 × [40" 94°C; 1' (45, 48, 50, or 55°C according to experiment), 2'72°C], 3'72°C

Fix50-60°C

3' 94°; 8 × [30" 94°C; 1' 50°C, 1'72°], 26 × [30" 94°C; 30"60°C; 1'72°C], 3'72°C

TD6

2'94°C; 14 × [1'94°C,1'58°C to 45°C (- 1°C/cycle), 1'72°C], 25 × [30"94°C, 45"58°C, 45"72°C], 3'72°C

TD6'

2'94°C; 14 × [1'94°C,1'58°Cto 45°C (- 1°C/cycle), 1'72°C], 25 × [40"94°C, 40"58°C, 1'72°C], 3'72°C

EE-GP

2' 94°; 35 × [20" 94°C; 1' 45°C; 1'72°]; 5' 72°C

DA

3' 94°C; 30 × [1' 94°C; 1' 45°C; 1'72°C]; 5' 72°C