From: An efficient method to find potentially universal population genetic markers, applied to metazoans
Genotyping technique | 454 sequencing | Cloning-sequencing | Melting curve genotyping | SSCP | Intron length polymorphism |
---|---|---|---|---|---|
Information (models of allele evolution) | Diploid Seq. (various) | Diploid Seq. (various) | Genotype (IAM)i | Genotype (IAM)i | Genotype (IAM)i |
Fragment size | Seq <400 bp* | No limit ** | Best <350 bp | Best <350 bp | No limit |
Allele number | No limit | No limit | Very limited. If high, impairs genotyping | Some alleles may not be distinguished | Some alleles may not be distinguished |
Throughput | high | low | high | Medium | medium |
PCR | Classical | Classical | Real Time | Classical | Classical |
Cloning | No | Yes | No | No | No |
Pooling loci | After careful quantification | Possible# but problematical | No | Possible | Common |
Electrophoresis | No | For sequencing | No | Yes | Yes |
Sequencing | 1 run = Numerous individuals × numerous loci | Several reactions required per individual | No | No | No |
Number human action steps μ | L + 1 + 1 | L + NL + 10NL | L | L + L/3 μ' | L + L/3 μ' |