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Table 1 Results of PCR amplification for each intron locus in each genus.

From: An efficient method to find potentially universal population genetic markers, applied to metazoans

Intron

1

2

3

4

5

6

7

8

9

11

12

13

14

Homolens version

II

II

II

II

II

II

II

II

II

II

II

II

II

Gene family (HBG-code)

004117

001043

003635

008731

052978

001266

006832

008594

052978

040291

040291

006550

005089

No primer pairs tested (without errors)

2

1

1

1

2

1

1

1

1

1

1

1

1

PCR size expected if intron absent

174

100

80

?

90-130

75

60

110

90

70

150

170

110

No genera 'P'

6

8

0

0

8

0

0

0

3

1

1

0

0

No genera 'I'

3

2

4

0

2

0

1

4

5

7

7

1

1

No genera 'A'

1

0

0

0

0

0

0

0

1

0

0

0

0

Paracentrotus

I

P

  

P

   

P

I

I

  

Amphipholis

P

P

I

 

P

 

I

 

P

I

  

I

Echinocardium (all conditions)

P

P

I

 

P

    

I

P

  

Corella

A

P

I

 

P

   

I

I

I

  

Perophora

P

P

  

P

   

I

P

   

Styela

P

P

  

P

   

P

I

I

  

Abatus (all species)

I

P

  

P

  

I

I

 

I

I

 

Sterechinus (all species)

P

P

  

I

  

I

I

 

I

  

Macoma

I

I

I

 

I

  

I

I

I

I

  

Cerastoderma

P

I

  

P

  

I

A

I

I

  

Corallium

P

I

na

na

I

na

na

na

I

na

na

na

na

Paramuricea

 

na

na

na

I

na

na

na

na

na

na

na

na

Intron

15

17

19

21

22

24

25

26

29

30

34

35

36

Homolens version

II

II

III

III

III

III

III

III

III

III

III

III

III

Gene family (HBG-code)

000163

003635

000144

001043

001043

001043

001601

001601

002428

002428

002428

002428

005337

No primer pairs tested (without errors)

1

1

2

4

1

2

4

4

1

1

2

4

2

PCR size expected if intron absent

170

135

120-135

120

150

180

193-210

180

165

155

155-165

120-155

125-145

No genera 'P'

3

0

2

5

2

0

5

0

5

2

3

0

4

No genera 'I'

6

0

0

5

3

0

5

1

3

2

2

0

4

No genera 'A'

1

0

4

0

3

0

0

0

0

6

1

0

0

Paracentrotus

I

 

P

P

P

 

I

 

P

P

P

  

Amphipholis

I

  

I

I

 

I

 

I

A

  

I

Echinocardium (all conditions)

I

  

P

P

 

I

 

P

I

   

Corella

I

  

I

I

 

P

 

I

A

  

I

Perophora

P

 

P

P

  

P

  

A

  

P

Styela

I

  

I

  

P

 

I

I

A

 

P

Abatus (all apecies)

P

 

A

P

A

 

I

 

P

P

P

 

P

Sterechinus (all species)

A

 

A

P

I

 

P

 

P

A

I

 

I

Macoma

I

 

A

I

A

 

P

I

P

A

I

 

I

Cerastoderma

P

 

A

I

A

 

I

  

A

P

 

P

Corallium

I

na

na

I

I

na

P

na

I

na

na

na

 

Paramuricea

I

na

na

na

na

na

na

na

na

na

na

na

 

Intron

37

38

39

40

41

42

43

44

45

46

47

48

49

Homolens version

III

III

III

III

III

III

III

III

III

III

III

III

III

Gene family (HBG-code)

007493

032846

032846

010911

010054

009263

039475

015649

015649

026608

026608

011376

011376

No primer pairs tested (without errors)

1

1

1

1

2

1

4

1

1

1

1

1

4

PCR size expected if intron absent

125

160

95

180

255

140

110-145

135

150

75

215

285

240-260

No genera 'P'

4

1

2

0

0

0

5

1

1

1

0

4

0

No genera 'I'

2

2

0

0

2

4

2

1

4

3

1

5

0

No genera 'A'

0

1

0

0

0

0

0

3

0

0

2

0

0

Paracentrotus

 

A

P

 

I

  

A

 

I

   

Amphipholis

I

I

   

I

I

I

 

I

I

P

 

Echinocardium (all conditions)

P

 

P

  

I

P

A

   

I

 

Corella

     

I

P

P

I

  

I

 

Perophora

P

I

    

P

A

P

I

A

I

 

Styela

P

P

   

I

I

 

I

 

A

P

 

Abatus (all species)

I

   

I

 

P

  

P

 

P

 

Sterechinus (all species)

           

P

 

Macoma

      

P

 

I

  

I

 

Cerastoderma

P

       

I

  

I

 

Corallium

A

I

  

na

 

P

na

I

P

I

 

A

Paramuricea

I

I

  

na

 

I

na

 

I

I

  

Intron

50

51

52

53

54

55

56

57

58

ATPsαJ

ATPSαi2

EF3

EF4

Homolens version

III

III

III

III

III

III

III

III

III

    

Gene family (HBG-code)

011376

011376

031768

031768

031768

031768

031768

007173

007173

    

No primer pairs tested (without errors)

1

4

1

2

4

1

2

1

1

1

1

4

4

PCR size expected if intron absent

120

240

260

120

75

160

260

145

210

90

330

250-280

210-35

No genera 'P'

6

5

1

5

3

1

2

2

0

2

2

1

3

No genera 'I'

4

4

0

2

4

1

2

1

1

4

3

4

6

No genera 'A'

0

0

0

0

1

0

0

0

0

0

0

4

1

Paracentrotus

P

P

P

P

P

  

I

   

A

I

Amphipholis

P

I

 

I

P

    

I

I

I

I

Echinocardium (all conditions)

I

  

P

P

P

   

I

I

 

I

Corella

P

I

  

A

 

P

  

I

 

I

A

Perophora

P

P

  

I

 

I

  

I

 

P

I

Styela

I

I

  

I

 

I

  

P

 

A

I

Abatus (all species)

I

P

 

P

I

I

 

P

  

I

I

I

Sterechinus (all species)

P

P

 

P

I

  

P

  

P

I

P

Macoma

P

I

 

I

    

I

P

 

A

P

Cerastoderma

I

P

 

P

  

P

   

P

A

P

Corallium

I

P

A

P

I

A

A

 

na

na

na

na

na

Paramuricea

P

 

na

P

I

 

P

P

na

na

na

na

na

  1. Empty cells correspond to absence of amplification product or occurrence of primer dimer; "na" (not available) corresponds to introns not tested in the considered genus
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BMC Ecology and Evolution

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