Figure 6

Phylogeny of the ChNPS11/ETP/ChNPS12 subclade. Extracted from maximum likelihood (PhyML with WAG plus gamma substitution matrix) phylogeny of complete A domain dataset (Additional file 6B). Domain structure of each NRPS is shown to the right of species abbreviation and accession number. Orange highlighted A domains reflect corresponding A domain in the phylogeny. Orange branches = ChNPS11/ETP mod1 and blue = ChNPS12/ETP mod2 subfamilies. ChNPS11 is monomodular, while all other NRPSs in the ETP module 1 group are bimodular; all have complete A-T-C modules. The A domain from a M. oryzae NRPS;PKS (MG07803.6) also groups here. Members of the ChNPS12 subfamily show a diversity of C-terminal domains as described in text and Fig. 5. The group includes two putative NRPSs from the chytrid, B. dendrobatidis, two proteins with either an incomplete (MGG15248.6) or a degenerate (BC1G07441_07442.1) first module and monomodular bacterial proteins consisting of single A domains. ChNPS12 homologs in Basidiomycete NPS12 group 2 consist of proteins with single A domains which appear to lack additional C-terminal domains and are highly expanded in the basidiomycete Postia placenta.