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Table 2 Statistical results from experiments 2a-2c: fecundity and behavioural responses to alarm pheromone exposure.

From: Aphid reproductive investment in response to mortality risks

 

Day 1 offspring

Total offspring (Day 2-6)

2a: aphid line by EBF exposure

d.f.

F

P

d.f.

F

P

   Line

3, 189

0.40

0.7500

3, 171

7.81

< 0.0001

   Exposure

1, 189

0.06

0.8000

1, 171

0.54

0.4600

   Line*Exp

3, 189

1.13

0.3400

3, 171

0.77

0.5100

 

Day 1 offspring

Total offspring (Day 2-6)

2bi: symbiont by EBF exposure (5A)

d.f.

F

P

d.f.

F

P

   Symbiont

3, 147

10.49

< 0.0001

3, 127

1.80

0.1500

   Exposure

1, 147

0.26

0.6100

1, 127

0.04

0.8400

   Symb*Exp

3, 147

6.22

0.0005

3, 127

0.17

0.9200

 

Day 1 offspring

Total offspring (Day 2-6)

2bii: symbiont by EBF exposure (LSR1)

d.f.

F

P

d.f.

F

P

   Symbiont

1,76

11.03

0.0014

1,58

4.30

0.0430

   Exposure

1,76

0.65

0.4220

1,58

0.72

0.4000

   Symb*Exp

1,76

5.45

0.0220

1,58

2.86

0.0960

 

Day 1 offspring

Total offspring (Day 2-6)

2b iii :line by symbiont by EBF exposure

d.f.

F

P

d.f.

F

P

   Line

1, 151

2.44

0.1202

1, 128

0.53

0.4699

   Symbiont

1, 151

24.26

< 0.0001

1, 128

3.26

0.0732

   Line*Symb

1, 151

0.36

0.5495

1, 128

2.83

0.0947

   Exposure

1, 151

1.07

0.3015

1, 128

0.60

0.4417

   Line*Exp

1, 151

4.36

0.0384

1, 128

0.43

0.5135

   Symb*Exp

1, 151

0.005

0.7412

1, 128

0.94

0.3339

   Line*Symb*Exp

1, 151

9.69

0.0022

1, 128

3.52

0.0629

 

Proportion of time on plant

Proportion of time active

2c: symbiont by EBF behaviour (5A)

d.f.

χ 2

P

d.f.

χ 2

P

   Symbiont

3

11.38

0.7463

3

25.15

0.0007

   Exposure

2

176.29

< 0.0001

2

97.38

< 0.0001

   Symb*Exp

6

106.13

0.0753

6

6.90

0.5825

  1. ANOVA statistics of fecundity data for experiments where aphids from different clonal lines (2a: 5A, G3, G6, LSR1) or with different symbionts (2bi: no symbiont, Hamiltonella defensa, Regiella insecticola, Serratia symbiotica; 2bii: no symbiont, Regiella insecticola; 2biii: 5A and LSR1 with no symbiont, or Regiella insecticola) exposed to the alarm pheromone EBF or hexane as a control. (2c) GLM statistics of the proportion of time aphids were either on their plant, or active after exposure to EBF, hexane or water.