Figure 3

Nucleotide Percent Identity Plot (PIP) for the PDE4A gene isoform. PDE4A gene sequences from multiple species were aligned to human PDE4A using MultiPIP maker. Both gene duplicates are shown for the Trub PDE4A gene isoform. PIP output files were cropped to condense the alignment and display only the exons of interest. The 5' exons for the PDE4A long form splice variants PDE4A8, PDE4A1, PDE4A2, PDE4A3/10 are colored blue, while the super-short form PDE4A4 5' exon is colored cyan. Vertical dotted lines represent breaks in the nucleotide sequence, and nucleotide positions as referenced to Hsap PDE4A along the bottom scale. The range of nucleotide percent identity displayed (between 50% and 100%) is indicated in the upper right corner of the plot.