Strong reproductive barriers in a narrow hybrid zone of West-Mediterranean green toads (Bufo viridissubgroup) with Plio-Pleistocene divergence

Table 2 Percentage of potential hybrids detected in populations where hybrids are expected or likely to occur

		MtDNA		Nuclear DNA		Hybrids, based on
Population	N	B. balearicus	B. siculus	pure B. balearicus	pure B. siculus	NewHybrids	Diagnostic alleles	Cytonuclear disequilibrium
13 (Calatabiano)	16	15	1	14	0	2	1	1
	100%	93.75%	6.25%	87.50%	0%	12.50%	6.25%	6.25%
14 (Giarre)	25	17	8	25	0	0	2	8
	100%	68%	32%	100%	0%	0%	8%	32%
15 (Gravina)	26	13	13	0	26	0	4	13
	100%	50%	50%	0%	100%	0%	15.38%	50%
16 (Misterbianco)	24	0	24	0	24	0	0	0
	100%	0%	100%	0%	100%	0%	0%	0%
18 (Centuripe)	17	0	17	0	15	2	2	0
	100%	0%	100%	0%	88.24%	11.76%	11.76%	0%

MtDNA: Numbers and percentages of individuals containing either B. balearicus or B. siculus mtDNA. Nuclear DNA: Numbers and percentages of individuals assigned by STRUCTURE to either B. balearicus or to B. siculus. Hybrids based on: potential hybrids detected using a) NEWHYBRIDS software, b) diagnostic microsatellite alleles or c) cyto-nuclear disequilibrium.

ISSN: 2730-7182