Skip to main content
Figure 7 | BMC Evolutionary Biology

Figure 7

From: Interference with histidyl-tRNA synthetase by a CRISPR spacer sequence as a factor in the evolution of Pelobacter carbinolicus

Figure 7

Spacer #1 has similar effects on the amounts of hisS and hisZ RNA. The strains shown are G. sulfurreducens MA159(pMA35-0) with the hisS transgene and a CRISPR repeat without spacer #1 (diagonally striped bars); MA159(pMA35-2) with both the hisS transgene and two copies of spacer #1 (speckled bars); and MA159(pMA35-!) with the hisS transgene and a single mutated copy of spacer #1 (diamond-patterned bars). Reverse transcription was performed with either primer MA0329 for hisS or primer MA0442 for hisZ, and the amount of cDNA was quantified by QRT-PCR. The mean of three biological replicates is shown; error bars represent the minimum and maximum.

Back to article page