Table 3 List of primers
From: Molecular diversity of antimicrobial effectors in the oyster Crassostrea gigas
| Â | Sense primers (5'3') | Antisense primers (5'3') | ||
|---|---|---|---|---|
gene | Â | PCR amplification from cDNA and gDNA | ||
Cg-prp | Cg-prp2F | CACCATGTTCTCTCGGAGGA | Cg-prp3R | TCTCTTCATCAAAACAAAGTCG |
Cg-defm | Cg-defm5F | CCACTTTCTGGTTTGCTGAG | Cg-defU4R | TCTTGGTCAGATTCAGWCTGG |
Cg-defh | Cg-defh4F | CTACCAGTTGTTCATACAGAG | Cg-defU4R | TCTTGGTCAGATTCAGWCTGG |
Cg-bpi | Cg-bpi1F | CTACCAGTTGTTCATACAGAG | Cg-bpi1R | GGATTTAATATATCCGTCTTCTG |
Cg-actin | Cg-actin1F | CTTCACAATGGGAGATGAAGA | Cg-actin1R | GTAAACTCCTATCACAGCCAC |
| Â | Â | gene copy number estimation | ||
Cg-prp | Cg-prpUq1F | CCACCATGTTCTCTCGGAGG | Cg-prpUq1R | CTTGTAGAACGGGCTAGCAC |
Cg-defs | Cg-defUq1F | CTGCAGACATGGCTTTTGCTG | Cg-defUq1R | GTACATCTTGACCAGAGCGTG |
Cg-bpi | Cg-bpiq2F | AGATAGAAATAGGAATGGACGG | Cg-bpiq2R | GTTATAGATCCACGCTGCTCC |
Cg-actin | Cg-actq2F | CAGCTATGTAGGAGACGAG | Cg-actq3R | CACGGAGTTCATTGTAGAAGG |
| Â | Â | 5' and ' RACE | ||
Cg-defh | Cg-defh3'sp1 | GCTGGATTTGGGTGTCCG | race (dT) anchor | GACCACGCGTATCGATGTCGAC |
Cg-defh | Â | Â | Cg-defh5' sp1 | CACAGTAGCCCGCTCTACA |
Cg-defh | oligo (dT) anchor | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTV | Cg-defh5' sp2 | CGGACACCCAAATCCAGC |
Cg-defh | race (dT) anchor | GACCACGCGTATCGATGTCGAC | Cg-defh5' sp3 | CCATYTCTGCAGAAACCATC |
Cg-defm | Â | Â | Cg-defm5' sp1 | CATCTTAACCAGAGCGTGGC |
Cg-defm | oligo (dT) anchor | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTV | Cg-defm5' sp2 | GGACTTGCAGTGATTGTTGC |
Cg-defm | race (dT) anchor | GACCACGCGTATCGATGTCGAC | Cg-defm5' sp3 | CATCAGAAGGACAGCTAGTG |