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Table 2 Estimation of gene copy number of antimicrobial effectors and non immune gene by real time PCR

From: Molecular diversity of antimicrobial effectors in the oyster Crassostrea gigas

gene Sample Primer efficiency (E) Calculated copy number Estimated copy number
   Mean1 SD Mean2 SD  
Cg-bpi pDNA 2.01 ± 0.006    
  gDNA 2.06 ± 0.060 0.95 ± 0.10 1
Cg-prp pDNA 1.99 ± 0.008    
  gDNA 2.03 ± 0.040 12.59 ± 0.99 11 - 13
Cg-defs pDNA 2.02 ± 0.004    
  gDNA 2.03 ± 0.010 47.46 ± 6.4 3 41 - 53
Cg-actin pDNA 1.99 ± 0.030    
  gDNA 2.07 ± 0.040 168.04 ± 10. 4 156 - 176
  1. gDNA: genomic DNA from C. gigas
  2. pDNA: plasmid DNA used as standard
  3. 1 Mean of primer efficiency (E = 10-slope) from three technical replicates
  4. 2 Mean of three biological replicates
  5. SD: standard deviation