Figure 3

RT-PCR expression profile of the early zygotic kinesin light chain gene AaKLC2.1 and the ubiquitously transcribed paralog AaKLC1. Thirty cycles of PCR were performed on cDNA synthesized using total RNA isolated from different life stages and ovaries. Negative control reactions using primers for RPS7 and AaKLC2.1 with no reverse transcriptase were performed to check for genomic DNA contamination (not shown). RPS7-specific primers were used for a loading control (bottom). A) AaKLC2.1. Lanes 1-11, embryos collected at 0-1, 1-2, ... and 10-11 hr after egg deposition. Lane 12, 12-24 hr embryo; lane 13, 24-40 hr embryo; lane 14, 1-4th instar larvae; lane 15, 0-48 hr pupae; lane 16, ovary from newly emerged females (0-1 day old); lane 17, ovary from 3-4 day old females; lane 18, ovary from females 48 hr post blood meal; lane 19, carcass minus ovaries, 48 hr post blood meal; lane 20, ovary from females 72 hr post blood meal; lane 21, 0-5 day old males, lane 22, 0-5 day old females. Note the faint bands in lane 3 and 7 (2-3 hr and 6-7 hr embryo). B) AaKLC1. Lanes 1-15 (same as above); lane 16, 0-5 day old males; lane 17, 0-5 day old females; lane 18, ovary from females 48 hr post blood meal; lane 19, carcass minus ovaries, 48 hr post blood meal.