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Table 1 Primers and annealing temperatures used for PCR and sequencing in this study

From: Rampant historical mitochondrial genome introgression between two species of green pond frogs, Pelophylax nigromaculatus and P. plancyi

Gene

Primer

Primer Sequences (5'-3')

Annealing T

Reference

Cyt-b

B104(F)

AAC ATC TCT GCA TGA TGA AAC TTC GG

55°C

This study

 

B829(R)

AT TGA GCG AAG GAT GGC GTA GGC GAA

  

POMC

POMC-1(F)

GAA TGT ATY AAA GMM TGC AAG ATG GWC C

50°C

Wiens et al. [56]

 

POMC-2(R)

TAY TGR CCC TTY TTG TGG GCR TT

  
 

POMC-3(F)

TCT GCM GAR TCW CCY GTG TTT CC

50°C

 
 

POMC-4(R)

TGG CAT TYT TGA AAA GAG TCA T

  

TYR

TyrlB (F)

AGG TCC TCY TRA GGA AGG AAT G

50°C

Bossuyt and Milinkovitch [57]

 

Tyr1G(R)

TGC TGG GCR TCT CTC CAR TCC CA

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