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Table 2 Placement of Myotubularin Homologue Sequences into Phylogenetic Similarity Clusters

From: A phylogenetic survey of myotubularin genes of eukaryotes: distribution, protein structure, evolution, and gene expression

"M1"(+) "R3"(+) "R5"(-) "R6"(+) "R9"(-) "R10"(-)
0.998 ± 0.0005 0.998 ± 0.0005 0.958 ± 0.032 1.000 ± 0.000 0.990 ± 0.014 0.913 ± 0.118
84.8 ± 10.5 91.5 ± 15.0 87.5 ± 8.2 98.5 ± 1.3 95.3 ± 2.5 89.0 ± 19.4
MTM1_Hu MTMR3_Hu MTMR5_Hu MTMR6_Hu MTMR9_Hu MTMR10_Hu
MTMR1_Hu MTMR4_Hu DrMTMR5 MTMR7_Hu DrMTMR9 DrMTMR10
MTMR2_Hu N001631983 MTMR13_Hu DrMTMR7 N001625874 MTMR11_Hu
DrMTMR2 Tad64213 Nv109357 MTMR8_Hu Tad28926 MTMR12_Hu
N001636543   Tad51481 N001626810 M01749550C N001629628
Tad38469   M001750622 Tad56124   Tad54716
    M01746603C   
  1. Myotubularin homologue sequences from human ("Hu") and Danio rerio ("Dr") were obtained from the literature and database keyword searches. For the other species represented, candidate myotubularin sequences were obtained by searching the protein datasets of fully sequenced eukaryotic genomes, as detailed in Methods. The prefix "N" or "Nv" followed by a numeral denotes sequences from Nematostella vectensis, "Tad" followed by a numeral denotes sequences from Trichoplax adhaerens, and "M" followed by a numeral denotes sequences from Monosiga brevicollis, (except "Mbrevi5R3" which is also from Monosiga). The sequences were aligned as detailed in Methods. Phylogenetic trees were inferred as detailed in Methods. Shown are the composition and clade support for the six similarity groups previously identified [2, 3]. The plus and minus symbols (+,-) indicate the presence of enzymatically active or inactive catalytic loop sequence signatures, respectively. For each group, the number above the line indicates the Bayesian clade support (posterior probability), and the number below the line indicates the bootstrap support (out of 100 total replicates) in Maximum Likelihood (ML). Results presented are the mean (± SD) of four distinct alignments.
  2. Sequences Tad51955 and M01745983 consistently clustered together as a distinct "new clade". The mean Bayesian posterior probability was: 0.998 (± 0.0005) (n = 4). The mean bootstrap support in Maximum Likelihood (ML) was: 97.5 (± 0.577) (n = 4).
  3. Sequences Nv82921, Tad62282 and MBrevi5R3 could not be placed into a similarity group with confidence.