Alignment of ROCO Kinase Domain Sequences. Myotubularin homologue sequences were identified as detailed in Methods. The protein kinase domains of these sequences were identified by searching at the NCBI CDD [40, 41]. "EHI" refers to Entamoeba histolytica myotubularin proteins, "DDB" refers to Dictyostelium discoideum myotubularin proteins, "Hu" refers to Homo sapiens proteins. Reference protein kinase sequences were obtained from the literature and keyword search as follows: "HuLRRK2" (GenBank: NP_940980); "TAK1Hu" (PDB: 2EVA_A); "TESK1Hu" (GenBank: NP_006276.2); "MOS_Hu" (GenBank: NP_005363.1); "SRC_Hu" (Swiss-Prot:P12931); "ABL_Hu" (Swiss-Prot:P00519). The figure has arrows marking the positions of functionally important residues, as defined by Kannan et al. .