Figure 5

WC1 and CD163cα tyrosine-based motifs in the cytoplasmic domain. (A)Cytoplasmic domains were determined by ascertaining the transmembrane domain, using the DAS transmembrane server [70]. Proteins are labelled as described in Fig. 4, with the addition of bovine WC1 cytoplasmic domains [31]. The evolutionary history of 34 taxa was inferred using Bayesian analysis in MrBayes3.2 [65]. Markov chain Monte Carlo analysis was performed for 830,000 cycles, using 2 runs of 4 chains each, a temperature setting of 0.2, and an amino acid mixed model to approximate the posterior probabilities of trees, shown at branch nodes. The average standard deviation of split frequencies was 0.01, which was diagnostic of convergence at < 0.05. (B)Multiple alignment of the amino acids surrounding the tyrosine motif phosphorylated in WC1 (bold) with similar amino acid sequences from CD163 family members [35].