Figure 3
From: Evolution of the CD163 family and its relationship to the bovine gamma delta T cell co-receptor WC1
Expression profiles of bovine CD163 genes. (A) Bovine ex vivo PBMC (lane 1), ConA-activated PBMC (lane 2), mesenteric lymph node (MLN, lane 3), lung (lane 4) and intestinal epithelial lymphocytes (IELs, lane 5) were evaluated for expression of CD163A and CD163c-α by RT-PCR. GAPDH is shown for comparison. These results are representative of reactions (n = 3) performed for at least two animals. (B) Bovine total PBMC, γδ TCR+ cells, γδ TCR - cells, WC1+ cells, and WC1- cells were evaluated for CD163A, CD163c-α, and GAPDH expression by RT-PCR. Templates were serially diluted by a factor of three. These results are representative of two reactions.