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Figure 1 | BMC Evolutionary Biology

Figure 1

From: Evidence for an early evolutionary emergence of γ-type carbonic anhydrases as components of mitochondrial respiratory complex I

Figure 1

BN-PAGE, CI enzyme activity (panel A) and two-dimensional BN/SDS-PAGE (panel B) profiles. Panel A shows the characteristic Coomassie-stained BN-PAGE profile of Acanthamoeba mitochondrial complexes (left) and in-gel assay of these complexes (Acanthamoeba BN-PAGE lane from the same gel), identifying an NADH dehydrogenase activity at ~940 kDa (right). This band is considered to represent intact CI from Acanthamoeba. Panel B shows the protein profile observed after two-dimensional BN/SDS-PAGE of Acanthamoeba mitochondrial complexes resolved in panel A. A list of proteins identified in CI and CI* isolated from BN-PAGE bands is presented in Additional File 1. CI, intact complex I (940 kDa); CI* = CI subcomplex (820 kDa); CIII, complex III; CV, complex V.

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