9.0) optimal pH, more than two pH units higher than that of vertebrate AANATs. Conclusions The substrate selectivity profiles of bfAANATα and δ' are relatively broad, including alkylamines, arylalkylamines and diamines, in contrast to vertebrate forms, which selectively acetylate serotonin and other arylalkylamines. Based on these features, it appears that amphioxus AANATs could play several roles, including detoxification and biogenic amine inactivation. The presence of seven AANATs in amphioxus genome supports the view that arylalkylamine and polyamine acetylation is important to the biology of this organism and that these genes evolved in response to specific pressures related to requirements for amine acetylation."/>
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Table 3 Acetylation activity in different light/darkness cycles

From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

Lighting Conditions blAANAT activity at pH 8.5
(nmol/h/μg of total protein)
  Midday Midnight
LD 0.299 ± 0.046 0.117 ± 0.046
LL 0.234 ± 0.063 0.256 ± 0.045
DD 0.271 ± 0.028 0. 354 ± 0.032
  1. Acetylation activity under normal light/darkness changes (LD) compared with constant light (LL) and constant darkness (DD) conditions. The pH of the assay was 8.5; data are given as the mean ± S.E.M. (N = 3 to 6).