In toto hybridization of B. lanceolatum. Eggs and embryos were hybridized as indicated in the Methods section either with the α subtype (left column) or the δζ subtype (right column) probes. The central column in the image presents the result of a typical control obtained using a sense probe. In these studies, both α and δζ sense probes were used; neither produced labeling. Bar = 50 μm. For further details see Methods.