9.0) optimal pH, more than two pH units higher than that of vertebrate AANATs. Conclusions The substrate selectivity profiles of bfAANATα and δ' are relatively broad, including alkylamines, arylalkylamines and diamines, in contrast to vertebrate forms, which selectively acetylate serotonin and other arylalkylamines. Based on these features, it appears that amphioxus AANATs could play several roles, including detoxification and biogenic amine inactivation. The presence of seven AANATs in amphioxus genome supports the view that arylalkylamine and polyamine acetylation is important to the biology of this organism and that these genes evolved in response to specific pressures related to requirements for amine acetylation."/>
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Figure 9 | BMC Evolutionary Biology

Figure 9

From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

Figure 9

In toto hybridization of B. lanceolatum. Eggs and embryos were hybridized as indicated in the Methods section either with the α subtype (left column) or the δζ subtype (right column) probes. The central column in the image presents the result of a typical control obtained using a sense probe. In these studies, both α and δζ sense probes were used; neither produced labeling. Bar = 50 μm. For further details see Methods.

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