9.0) optimal pH, more than two pH units higher than that of vertebrate AANATs. Conclusions The substrate selectivity profiles of bfAANATα and δ' are relatively broad, including alkylamines, arylalkylamines and diamines, in contrast to vertebrate forms, which selectively acetylate serotonin and other arylalkylamines. Based on these features, it appears that amphioxus AANATs could play several roles, including detoxification and biogenic amine inactivation. The presence of seven AANATs in amphioxus genome supports the view that arylalkylamine and polyamine acetylation is important to the biology of this organism and that these genes evolved in response to specific pressures related to requirements for amine acetylation."/>
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Figure 5 | BMC Evolutionary Biology

Figure 5

From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

Figure 5

Enzyme activity of GST-bfAANATα and GST-bfAANATδ'. A. pH optimum. Data are presented as the percent of maximal activity for each protein. Data represent results of two independent experiments with the difference of means < 5%. For comparison, data for ovine and yeast AANAT from a previous study are shown [8]. B. Inhibition of GST-bfAANATα by bisubstrate inhibitor CoA-T. C. Acetylation activity of GST-bfAANATα towards tryptamine measured at different temperatures to determine the optimum. Data represent results of two independent experiments with the difference of means < 5%. Data in B are presented as the mean ± S.E.M (N = 3). For further details see Methods.

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