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Table 2 Sequence and location of all primers used to amplify parts of the mitochondrial genome

From: Concerted evolution of duplicated mitochondrial control regions in three related seabird species

Primer

Location

Sequence

Source

b2

5' end of cytochrome b

5'-GCCCCTCAGAATGATATTTGTCCTCA-3'

1

b3

middle of cytochrome b

5'-GGACGAGGCTTTTACTACGGCTC-3'

2

b4

middle of cytochrome b

5'-TTGCTGGGGTGAAGTTTTCTGGGTC-3'

2

b5

3' end of cytochrome b

5'-TTCCACCCCTACTTCTCACTAAAAGA-3'

2

cytb-endL

3' end of cytochrome b

5'-TATCATCGGCCAACTAGCC-3'

3

b6

tRNA-Threonine

5'-GTCTTCAGTTTTTGGTTTACAAGAC-3'

2

16363H

5' end of ND6

5'-GTTGTGACCGTTGATAGTG-3'

3

ND616363

5' end of ND6

5'-CACTATCAACGGTCACAAC-3'

3

tGlu-boobyH

tRNA-Glutamic Acid

5'-ACAACGGCGGCATTTCAGGCC-3'

3

SsMCR-L143B

5' end of CR1

5'-ATTGCACATTARATTTASCT-3'

3

SsMCR-H170B

5' end of CR1

5'-ATGAAAGTATTATGTGATCC-3'

3

SlMCR-L160B

5' end of CR1

5'-ATCCACATTGCACATTAAGT-3'

3

SlMCR-H171B

5' end of CR1

5'-CATCAATTTACATATGTCGAC-3'

3

SdMCR-H750

middle of CR1 and CR2

5'-GGGAACCAAAAGAGGAAAACC-3'

4

SlMCR-L740

middle of CR1 and CR2

5'-GCATAGGAAGTACTTACAATCTAGG-3'

3

SsMCR-L143A

5' end of CR2

5'-ATTGCACATTARATTTAYTC-3'

3

SsMCR-L143A2

5' end of CR2

5'-ATTGCACATTAAATTTAGTC-3'

3

SsMCR-H169A

5' end of CR2

5'-TGAAAGTATACAGTCCATTG-3'

3

SlMCR-L160A

5' end of CR2

5'-ATCCACATTGCACATTTAAA-3'

3

SlMCR-H194A

5' end of CR2

5'-CAGTGGTATGTGTTTGTC-3'

3

12SH1301

5' end of 12SrRNA

5'-GGTAAGGTTAGGACTAAGTC-3'

3

  1. Source refers to the original source of the primer sequence (1 = Kocher et al. [33], 2 = T. Birt unpublished, 3 = this study, 4 = Steeves et al. [34]). See Figure 1 for a graphical representation of primer locations.