Figure 3

Teleosts display a conserved number of retained slc1 genes. For this analysis the following species in addition to mouse (mm) and human (hs) were used: Zebrafish Danio rerio (dr), Takifugu rubripes (tr), Medaka Oryzias latipes (ol) and the stickleback Gasterosteus aculeatus (ga). The phylogenetic tree was build using the maximum likelihood method on a 331aa stretch determined by the program Gblocks after MUSCLE alignment of the full-length SLC1 amino acid sequence. Bootstrap values above 50% (0.5) are shown. While zebrafish slc1 genes are shown in dark red, other teleost slc1 genes are highlighted in light red. Within the teleost family the number and phylogeny of slc1 genes is highly preserved, with the exception of slc1a7b/eaat5b which could neither be found in stickleback nor in torafugu. In case of slc1a9 the takifugu rubripes gene was replaced by the Tetraodon gene as the takifugu slc1a9 gene could only be partly assembled. Note that teleost slc1a2c/eaat2c and slc1a7c/d/eaat5c/d genes are phylogenetically clearly separated from other SLC1 subfamilies pointing towards the existence of two additional species specific subfamilies now called slc1a8 and slc1a9. The scale bar shows the percentage of amino acid substitutions required to generate the corresponding tree.