Standard | Serial Con | CT | Cultivation: | |
---|---|---|---|---|
Plasmid (Hmo_41) | 0.2 μg/μl | 11.10 | Y = -0.2989x+14.22 | |
 | 0.02 μg/μl | 16.30 | Y = -0.2989x+14.22 | |
 | 0.002 μg/μl | 18.36 | R ^2 = 0.997; E = 0.99 | |
 | 0.0002 μg/μl | 21.72 | Results: plasimid DNA size = 2× 103 bp copy number of plasmid per μl = 6.5× 1011 (2 ug/ul) | |
 | 0.00002 μg/μl | 25.30 |  |  |
Sample | Con/(ug/ul) | CT | PCNH | Avg. |
Xenocypris argentea | 0.115 | 13.32 | 183221.1127 | 3.0 × 105 |
 | 0.0115 | 15.44 | 425899.8786 |  |
Cutler alburnus | 0.9 | 12.12 | 53470.08164 | 3.5× 104 |
 | 0.09 | 17.11 | 17242.48462 |  |
Elopichthys bambusa | 0.133 | 15.32 | 39996.47484 | 4.0 × 104 |
 | 0.0133 | 18.69 | 39330.43191 |  |
Squaliobarbus curriculus (Genome size≈ 1 pg) | 0.1 | 13.83 | 148331.8306 | 1.7 × 105 |
 | 0.01 | 16.8 | 192088.4962 |  |
Mylopharyngodon piceus (Genome size≈ 1 pg) | 0.36 | 15.04 | 17916.89943 | 1.7× 104 |
 | 0.036 | 18.46 | 17022.5593 |  |
Ctenopharyngodon idellus (Genome size≈ 1 pg) | 0.314 | 15.32 | 16941.18202 | 1.7 × 104 |
 | 0.0314 | 18.69 | 16659.06829 |  |
Leuciscus tumensis | 0.104 | 13.32 | 202600.2689 | 3.3× 105 |
 | 0.0104 | 15.44 | 470946.9811 |  |
Saurogobio dabryi | 0.03 | 11.91 | 1853536.326 | 1.7× 106 |
 | 0.003 | 15.51 | 1555826.276 |  |
Rhodeus ocellatus (Genome size≈ 1 pg) | 0.092 | 13.91 | 152592.9712 | 2.2× 105 |
 | 0.0092 | 16.23 | 309091.1208 |  |
Cyprinus carpio (Genome size≈ 2 pg) | 0.0033 | 22.06 | 31174.78108 | 3.1× 104 |
 | 0.00033 | 25.39 | 31511.30668 |  |
Shizothorax grahami | 0.08 | 10.66 | 3286149.864 | 5.1× 106 |
 | 0.008 | 12.87 | 7179899.5 |  |