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Table 4 Rate tests on tubulin orthologs.

From: Tubulin evolution in insects: gene duplication and subfunctionalization provide specialized isoforms in a functionally constrained gene family

Beta 1

Mean Chi-Sq. Vs. Orthologs

(n = 8)

Beta 2

Mean Chi-Sq. Vs. Orthologs

(n = 8)

Beta 3

Mean Chi-Sq. Vs. Orthologs

(n = 8)

Beta 4

Mean Chi-Sq. Vs. Orthologs

(n = 4)

Dmβ1

1.08 +/- 0.91

Dmβ2

1.02 +/- 2.09

Dmβ3

0.71 +/- 0.70

Dmβ4

1.62 +/- 1.72

Aeβ1

1.49 +/- 1.51

Aeβ2

1.47 +/- 3.28

Aeβ3

1.61 +/- 1.46

Aeβ4a

1.29 +/- 0.44

Agβ1

0.96 +/- 0.98

Agβ2

5.54 +/- 2.11

Agβ3

1.19 +/- 1.20

Agβ4

1.68 +/- 1.65

Bmβ1a

1.65 +/- 1.05

Bmβ2

1.01 +/- 1.42

Bmβ3

2.31 +/- 1.89

Apβ4

2.53 +/- 1.49

Tcβ1

1.19 +/- 1.58

Tcβ2a

1.56 +/- 2.47

Tcβ3

2.82 +/- 1.75

Phβ4

29.84 +/-24.42

Amβ1

0.97 +/- 0.98

Amβ2a

1.50 +/- 1.94

Amβ3

1.44 +/- 1.22

  

Nvβ1

0.74 +/- 1.09

Nvβ2a

1.25 +/- 2.06

Nvβ3

1.48 +/- 1.35

  

Apβ1

2.18 +/- 2.17

Apβ2a

0.57 +/- 0.93

Apβ3

2.69 +/- 1.81

  

Phβ1

0.51 +/- 0.44

Phβ2a

0.18 +/- 0.16

Phβ3

5.91 +/- 3.03

  

Alpha 1

Mean Chi-Sq. Vs. Orthologs

(n = 8)

Alpha 2

Mean Chi-Sq. Vs. Orthologs

(n = 4*)

Alpha 3

Mean Chi-Sq. Vs. Orthologs

(n = 1)

Alpha 4

Mean Chi-Sq. Vs. Orthologs

(n = 5)

Dmα1a

0.29 +/- 0.23

Dmα2

2.37 +/- 3.34

Bmα3

0.56

Dmα4

7.29 +/- 10.41

Aeα1a

1.42 +/- 0.98

Aeα2

4.76 +/- 0.60

Tcα3

0.56

Agα4a

5.46 +/- 5.83

Agα1a

0.48 +/- 0.42

Bmα2

1.69 +/- 2.91

  

Tcα4

13.31 +/- 18.91

Bmα1

0.74 +/- 1.05

Tcα2

1.51 +/- 2.31

  

Amα4

22.17 +/- 11.79

Tcα1

0.43 +/- 0.71

Amα2

56.30 +/- 6.55

    

Amα1a

0.45 +/- 0.69

Phα2

1.63 +/- 1.72

    

Nvα1a

0.29 +/- 0.25

      

Apα1a

0.44 +/- 0.44

      

Phα1a

0.83 +/- 1.05

      
  1. Protein evolutionary rates were compared among tubulin orthologs. The average +/- Sdv of chi-square values between each tubulin isoform and its insect orthologs is presented (eg. Dmβ1 = (Chi-sq. Dmβ1 vs. Agβ1 + Chi-sq. Dmβ1 vs. Aeβ1 +...)/8), using Pediculus humanus corporis orthologs as outgroups; Pediculus rates tested with Acyrthosiphon pisum outgroups. In taxa with multiple copies of an isoform, the conserved isoform is used in the rate test. *The highly divergent Amα2 isoform is an outlier, and was removed from the analysis. Chi-Sq. values > 3.8 have a probability of p < 0.05; Chi-Sq. values > 5.2, p < 0.01.