Major isoform | Minor isoform | Rate test (mean Chi-Sq. +/- Sdv) |
|---|
Insect β1 | Insect β2 | 5.12 +/- 3.81 | n = 9 |
Insect β1 | Insect β3 | 11.85 +/- 4.33 | n = 9 |
Insect β1 | Insect β4 | 21.34 +/- 21.15 | n = 6 |
Insect α1 | Insect α2 | 10.66 +/- 17.34 | n = 6 |
Insect α1 | Insect α3 | 71.19 +/- 9.91 | n = 2 |
Insect α1 | Insect α4 | 97.87 +/- 62.34 | n = 4 |
- Protein evolutionary rates were compared between major and minor tubulin paralogs resulting from ancient duplication events predating separation of insect orders, using Ha major alpha and beta tubulins as outgroups. The average and standard deviation of chi-square values for pairwise rate tests are presented (eg. Insect β1 vs. Insect β2 = (Chi-sq. Dmβ1 vs. Dmβ2 + Chi-sq. Agβ1 vs. Agβ2 + Chi-sq. Aeβ1 vs. Agβ2...)/9). In taxa with multiple copies of an isoform, rate tests are performed using the conserved isoform. The only Drosophila species included is Dm, to avoid a Dipteran skew in the results. Chi-Sq. values > 3.8 have a probability of p < 0.05; Chi-Sq. values > 5.2, p < 0.01.
