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Table 1 Primers used for sequencing and for determining in which clade alleles fall.

From: A Δ11 desaturase gene genealogy reveals two divergent allelic classes within the European corn borer (Ostrinia nubilalis)

Primer name

Purpose

Sequence

403f

amplification and sequencing

GCTGTATTTGGGATTTACATCAG

781r

amplification and sequencing

TGGAACGCTTTGTTTCGTTCTC

550f

sequencing

CATRGTGTTTAACAGCATGGC

I267f

sequencing

AATACCCTCWGCAAAAGTCTA

ExonAf

A clade specific

TTATGGAGCCACAGAAGCTAC

ExonBf

B clade specific

CTATGGAGTCATAGAAGCTAT

IntronAr

A clade specific

GTCGGTCATTGGGTGTTTGTAG

IntronBr

B clade specific

GCCGGTCTAGTTGTGCTTGTAG

  1. Mismatches between clade specific primers are in bold.