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Table 1 Primers used for amplification and sequencing

From: Allopolyploid speciation and ongoing backcrossing between diploid progenitor and tetraploid progeny lineages in the Achillea millefoliumspecies complex: analyses of single-copy nuclear genes and genomic AFLP

Primer name

Primer sequence

Reference or source

GScp687f

5'-GATGCTCACTA CAAGGCTTG-3'

[40]

GScp994r

5'-AATGTG CTCTTTGTGGCGAAG-3'

[40]

GS-f

5'-AACCAATGGAGAAGTTATGC-3'

this study

GS-r

5'-CAAAACCACCTTCTTCTCTC-3'

this study

AA11F

5'-TTY GCN TTY TGG GAY TGG GT-3

[45]

Yamv (reverse)

5'-TCI ACI CCC CAI TGR TCA AAI GAR TTI AT-3'

[45]

PgiC-11F

5'-TY TGGGAYTGGGTAGGAG-3'

this study

PgiC-14F

5'-GAGTGTATGGAATGTCTC-3'

this study

PgiC-21R

5'-GGARTTGATTCCCCAAAC-3'

this study