From: The mammary gland-specific marsupial ELP and eutherian CTI share a common ancestral gene
ELP/CTI gene/ transcript | Name | Primer Sequence1 5' 3' | PCR Product Size (bp) | Primer Conditions |
---|---|---|---|---|
FT dunnart transcript | FT_ELP_F | GTCAAGTGTTATCTACTGGCAGCACCATG | 488 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 59°C for 30 sec; 68°C for 1 min; 68°C for 10 min |
 | FT_ELP_R | CCCAAAGTGCTGTTAATGCTTTATTGTAGC |  |  |
Opossum transcript | mELP_NheI_F | GCTAGCAAGGTTTTCTCTCAGTGCCATC | 488 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 60°C for 30 sec; 68°C for 30 sec; 68°C for 10 min |
 | mELP_BamHI_R | GGATCCTGTTAATGCTTTATTGTACCAG |  |  |
Tammar transcript | tELP_NheI_F | GCTAGCAAGTGTAGTCTACCAGTGGCACC | 479 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 58°C for 30 sec; 68°C for 30 sec; 68°C for 10 min |
 | tELP_BamHI_R | GGATCCTGTTAATGCTTTATTGTACCAG |  |  |
Dog transcript | Dog_ELP_Ex1_F | GCCTAGAACATTCAGCTATTGGCACC | 449 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 55°C for 30 sec; 68°C for 1 min; 68°C for 10 min |
 | Dog_ELP_Ex3_R | TGAATGTTTTATTGACCTAGACCTGGAGG |  |  |
Cow transcript | bELP_NheI_F | GCTAGCAACTCACAGCTCCTCACACCATG | 463 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 58°C for 30 sec; 68°C for 30 sec; 68°C for 10 min |
 | bELP_BamHI_R | GGATCCGAACACTTTATTGACCCAGTCCTG |  |  |
FT dunnart gene | FT_ELP_F | GTCAAGTGTTATCTACTGGCAGCACCATG | 4771 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 55°C for 30 sec; 68°C for 6 min; 68°C for 10 min |
 | FT_ELP_R | CCCAAAGTGCTGTTAATGCTTTATTGTAGC |  |  |
Koala gene | tELP_Ex1_F | GGTAGCAAGTGTAGTCTACCAGTGGCACC | 1428 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 52°C for 30 sec; 68°C for 4 min; 68°C for 10 min |
 | tELP_BamHI_R | GGATCCTGTTAATGCTTTATTGTACCAG |  |  |
Tammar gene (6.2 kbpromoter) | T7 | TAATACGACTCACTATAGGG | 6326 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 57°C for 30 sec; 68°C for 8 min; 68°C for 10 min |
 | tELP_Prom_R | GACTGATCAGACCAATATAAGCTT |  |  |
Tammar gene (7.9 kbpromoter) | T7 | TAATACGACTCACTATAGGG | 8044 | 94°C for 2 min; 35 cycles of 94°C for 30 sec; 57°C for 30 sec; 68°C for 8 min; 68°C for 10 min |
 | tELP_Ex1_R | GAGGGCCAACGATGGTAAATTTCAT |  |  |